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The following monoclonal antibodies was in fact bought off Pharmingen (San diego, Calif
Telephone staining and you may disperse cytometry. Single-cell suspensions were prepared from different lymphoid organs and incubated for 10 min at 10 6 cells/20 ?l on ice in staining buffer (phosphate-buffered saline [PBS] containing 0.5% bovine serum albumin [BSA] and 0.01% NaNstep 3) with optimal…